Regulatory

Part:BBa_J119414:Design

Designed by: Isabella Wiebelt-Smith   Group: Eckdahl Lab   (2018-11-07)


TM1 - pTac Tm control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence couldn't have the potential to form any secondary structures, so the Gs and Cs had to be alternated without creating any consecutive runs of one base.


Source

This base comes from flipping the bases of the promoter sequence in BBa_J119412 (excluding the -35 and -10 regions, which remained the same as those in the pTac promoter).

References